Laboratory of Photosynthesis
Laboratory of photosynthesis deals with the basic research of microbial photosynthesis with extension to the applied research concerning construction of photosynthetic measuring devices. The important characteristics of the laboratory is the multidisciplinary approach which covers disciplines from biophysics, molecular biology, biochemistry up to physiology and classical microbiology. There are four principal topics which are studied in particular groups led by a single senior scientist:
Mostly using a model cyanobacterium Synechocystis PCC 6803 it deals with mechanisms and regulation of the assembly of membrane pigment-protein complexes and their quality control. It focuses on the role of structural subunits and auxiliary protein factors in the process of Photosystem II assembly and on the elucidation of structure and function of homologues of the bacterial proteases FtsH in the degradation of structural as well as regulatory proteins participating in the biogenesis of photosynthetic apparatus..
Ecophysiology of algae - Ondřej Prášil
It analyzes molecular mechanisms of photosynthesis in various photothrops (cyanobacteria, algae) on different level of complexity - from ecophysiology studies with whole population (physiological and spectroscopical measurements in open oceans) to a single protein level (biophysical measurements with single cell, with proteins). It is interested in processes regulating photosynthesis, namely in mechanism of non-photochemical quenching, role of diazotrophy in cyanobacteria, mechanism of proteins mobility in thylakoid membrane and allocation of photosynthetic carbon into metabolic processes.
Biosynthesis and function of photosynthetic pigments - Roman Sobotka
It deals with the study of chlorophyll biosynthesis pathway, which is rather complicated and partially overlap with the pathway for synthesis other important cofactors like heme and phycobilins. The aim of the work is to elucidate mechanisms of regulation of chlorophyll biosynthesis and to identify pathways by which the chlorophyll is built in membrane pigment-protein complexes and by which is removed from them during their degradation.
Dynamics of Photosynthetic Membranes - Radek Kaňa
It studies mobility and photoprotection of photosynthetic proteins. We use single-cell microscopic methods (FRAP, FCS) to resolve process of proteins diffusion and reorganization in thylakoid. We also study mechanism of photosynthetic energy conversion in light-harvesting antennae, how it is optimized by non-photochemical quenching (NPQ) or during state transitions.